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PHD ULTRA™Series MA1 70-30xxPHD ULTRA™Syringe Pump SeriesUser's GuidePublication 5419-002-REV-APATENTS PENDING

Quick Guide10 THINGS YOU NEED TO KNOW TO USE THE PHD ULTRA™Since many of our users are already familiar with the operation of a syringe pump, we’ve cr

Syringe Loading – Micro Liter Multi-RackFigure 82: Front view of PHD ULTRA™showing controls for micro liter multi-rack syringe loading.1. Squeeze Rele

Figure 83: Micro Liter Multi-Rack Configuration.Syringe Loading – Push-Pull MechanismFigure 84: PHD Ultra showing Push-Pull Mechanism installed.To loa

Figure 85: PHD Ultra showing syringes installed on both sides of the Pusher Block.Remote Unit ConnectionConnect the control unit to the remote pump un

APPENDIX E: EXTERNAL CONNECTIONSUser I/O Connector SpecificationsPPiinn AAssssiiggnnmmeennttss1. Direction control inputRising edge sets pump to infu

Analog InputFigure 87: 4-Pin miniDIN FemaleAnalog control of the motor speed can be achieved in many ways. The following circuit is recommended.A DC

USB Figure 89: USB Type B FemaleUSB VIRTUAL COMMPORT DRIVER INSTALLATIONWhen you connect the PHD Ultra pump to a computer via USB, Windows will seek t

2. Choose “Install from a list or specific location (Advanced)” and click Next.3. Browse for the proper driver installation file and then click next.

RS-232 SpecificationsFigure 90: RS-232 9-Pin D-Sub MaleFigure 91: RS-232 Specifications.Baud Rates:9600192003840057600115200Word size – 8Parity – None

Legacy RJ-11 OptionFigure 92: Pumps with Legacy RJ-11 Option.Figure 93: Daisy-Chaining with Legacy RJ-11 Option. ✐NOTE: Pumps 1...n also have RJ-11 te

RS-485Figure 94: RS-485 In/Out - IEEE-1394 SocketsFigure 95: Daisy -Chaining via RS-485.Remote Pump ConnectionFigure 96: 14-POS, Circular Plastic Fema

General InformationCONVENTIONS USED IN THIS GUIDEThis guide describes basic procedures for operating the PHD ULTRA™Syringe Pump, including bothhardwar

APPENDIX F: MAINTENANCEMaintenancePHD ULTRA™series pumps require no special maintenance other than keeping them clean by avoidingaccidental spills of

Fuse ReplacementBattery Replacement1. Remove screw and swing cover to theside.2. Slide battery out from under clip.3. Install Lithium Coin Battery: 3V

Upgrading EZ PRO Software1. Upload the latest software version to your desktop (format is filename.dfu).2. Connect the pump to PC using a USB cable.

TroubleshootingInfusion Accuracy To ensure infusion accuracy always use new syringe(s) and measure syringe bore diameter and enter actualdimensions in

PHD ULTRA™Display Error MessagesMotor Stalled This indicates that the pusher block travel has been impeded. This may be caused by the syringe plunger

APPENDIX G: ‘AUTO FILL’ VALVESThere are two 'Auto Fill' Valve models available:Catalog No. Product55-7002 Supplied with 1/8 inch ID, 1/4 inc

APPENDIX H: ORDERING INFORMATIONStandard VersionDescription Stand Alone RemotePHD ULTRA™Infuse Only 70-3005 70-3105PHD ULTRA™Infuse/Withdraw 70-3006 7

AccessoriesCatalog # Description70-4000 RS-485 Cable for Pump-to-Pump Communication, 0.5m70-4001 RS-485 Cable for Pump-to-Pump Communication, 2m70-400

Declaration of ConformityManufacturer: Harvard Apparatus, Inc.84 October Hill RoadHolliston, Massachusetts 01746-1388, U.S.A.Phone: (508) 893-8999We

Harvard Apparatus pumps are distributed on a non-exclusive basis by: Instech Laboratories, Inc. Headquarters: 5209 Militia Hill R

WARRANTY AND REPAIR INFORMATIONManual DescriptionThis manual is designed to provide all operational and program information required to operate andmai

SAFETY INFORMATIONPlease read the following safety precautions to ensure proper use of your syringe pump. If the equipmentis used in a manner not spe

Introduction To The PHD ULTRA™PumpPRODUCT OVERVIEW – THEORY OF OPERATIONThe PHD ULTRA™Syringe Pump series is a family of high-accuracy, microliter- an

SPECIFICATIONSHarvard Apparatus PHD ULTRA™Syringe Pump Series User's ManualPublication 5419-002-Rev-A15Introduction To The PHD ULTRA™PHD ULTRA™S

Setting Up The PHD ULTRA™With EZ PRO™PHYSICAL VIEWSThe following diagrams show the important components of the PHD ULTRA™pump.Figure 1: Rear view of

NAVIGATING THE PHD ULTRA™EZ PRO™SOFTWAREThe PHD ULTRA™uses a high resolution touchscreen with an icon-enabled graphical user interface and anadvanced

The PHD ULTRA™supports touchscreen orientation auto-rotation (landscape/portrait) for permitting easyuse of the pump when positioned upright on its si

IN FORMATION /WA RNIN G ME SSA GESThe Message Area of the touchscreen is used to display helpful instructions for the currently displayedscreen. It is

Quick Guide...1010 Things You Nee

EZ PRO Touchscreen Button ReferenceThe following buttons are part of the PHD ULTRA™software:SSeettttiinnggss: Access the settings menu usedto adjust

SYSTEM SETTING ADJUSTMENTSThe PHD ULTRA™Settings menu is used to control basic system operations. This section describes theoptions available in this

TO SET THE USER-CONFIGURABLE AUDIBLE ALARMS:1. In the Audible Alarms menu, select the specific alarm or alarms that you wish to set. On the Settingssc

Locking The Touchscreen The PHD ULTRA™incorporates a Touchscreen Lock function that can be enabled to prevent accidentaloperation or interruption of o

TURNING TOUCHSCREEN LOCK OFFTo turn Touchscreen Lock off while running a Method, follow these steps:1. From the Method Run display, the Touchscreen L

Setting the Time and DateThe PHD ULTRA™displays the date and time on each screen in the user interface. Built-in battery backupsmaintain the date and

Setting Backlight LevelYou can adjust the backlighting level on the PHD Ultra touchscreen to optimize viewing in your specificlighting conditions. On

Setting the RS-232 Baud RateFor attaching the PHD Ultra to an external device using RS-232 serial communications, you can easilyadjust the baud rate

Maintenance/Calibration RemindersThe PHD Ultra monitors usage and provides convenient reminders for periodic maintenance or calibration.You can adjust

Power Up RunningThe Power Up Running Setting is designed to allow the pump to restart pump operation automaticallyafter a power outage when operating

Maintenance/Calibration Reminders ...28Power Up Running ...

SYRINGE LOADINGFigure 18: Front view of PHD ULTRA™showing important controls for syringe loading.1. Adjust the Pusher Block (2) to the approximate len

Figure 20. Syringe fully mounted and locked in place. Note mechanical stops set in position to prevent excess travelon pusher block.Changing the brack

Using The PHD ULTRA™PUMP OPERATION OVERVIEWThe following diagram provides an overview of basic operation for the PHD ULTRA™:OVERVIEW OF METHODSTo oper

• Multiple infusions• Ramped or stepped infusion rates• Periodic dispensing loops• Combination infuse and withdraw profiles•External system control or

RUNNING THE PUMPControlling The Pump The software provides Fast Forward (Infuse) and Fast Reverse (Withdraw) buttons that can be used tomanually contr

Figure 23: Method Main screen showing a sample Method with 10 steps.SY RINGE S ELEC TIO NIn order to accurately control the flow rate or volume delive

2. From the Syringe Selection menu, double tap the Manufacturer/Model button for the desired syringeto enter the Syringe Size Selection screen.Figure

Controlling The Pump Using A Stored MethodThe PHD ULTRA™Methods contain the detailed parameters required to control the pump for infuse orwithdraw ope

PREVIEW THE METHOD7. To Preview a Method, press the Method Preview button to enter the Run (Quick Start Methods) orMethod Run (Preloaded and User-Defi

RUN THE METHOD9. To start a pump Method, choose the Method Run button. While a Method is running, the Pump RunLED will be lit. The Run/Method Run scr

Concentration Profile ...63Autofill Profi

Figure 32: User-Defined Method Run screen with Method paused on Step 1. Note the currently active Step ishighlighted.11. To Resume a Method that is pa

USING AN EZ PRO QUICK START METHODQuick Start Methods are designed to help you quickly and conveniently use the PHD ULTRA™for simpleinfusion or withdr

2. Choose the Syringe Select button to enter the Syringe Selection screen. 3. Follow the instructions in the Syringe Selection section of this Guide t

Figure 36: Set Infuse and Withdraw Rates in a Quick Start Infuse and Withdraw combined Method.TO SET THE INFUSE RATE (INFUSE ONLY METHOD):1. From the

TO SET THE TARGET VOLUME OR TIME:1. From the Quick Start screen, choose the Target Volume/Time Select button.2. On the Set Target Volume or Time scree

Preloaded/User-Defined Method Workflow The following flow diagram describes the use of an existing Preloaded or user-Defined Method:Figure 38: Preload

STEP-BY-STEP OPERATION EXAMPLE: USING A PRELOADED OR USER-DEFINED METHODThe following steps describe operation using a Preloaded or User-Defined Metho

Setting A Time Delay For Method OperationWhen using a Preloaded or User-Defined Method, you can set the PHD Ultra to run the Method at a specificdate

CREATING AN EZ PRO NEW METHODIf none of the Methods stored on the PHD ULTRA™match your requirements, you can create a new Methodand store the Method f

4. When you have entered the desired name for your Method, choose the Accept button to return to theMethod Main screen. Your new Method will now be l

Appendices ...89Appendix A: Syrin

Modifying a Preloaded/User-Defined MethodOnce you have created a Method or have reviewed a Preloaded Method, you may have a need to modifythe structur

DEFINING METHOD STEP PARAMETERSThis section of the User Guide describes each of the available functions available for use in PHD ULTRA™Preloaded and U

Pump Operation ProfilesThe PHD ULTRA™Preloaded Methods and all User-Defined Methods use Pump Operation Profiles as part ofthe Methods controlling the

CO NSTANT RATE P ROFI LEThe Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode(infuse or withdraw) u

6. When all parameters are correctly set on the Set Rate screen, choose Accept to confirm your settingsand return to Step Definition screen. 7. Choose

RA MP PROF ILEThe Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumpingmode (infuse or withdraw) over

Figure 50: Set Start and End Rates screen.3. Select the Start Rate button (it should be selected by default as indicated by the active data entry boxt

ST EPP ED P ROFILEThe Stepped Rate profile is used to create an increasing or decreasing flow rate that varies in a series ofequal steps between a sta

Figure 52:. Set Start and End Rate screen. 3. Select the Start Rate button (it should be selected by default as indicated by the active data entry bo

PU LSE PRO FILEThe Pulse Rate profile is used to create an oscillating infusion or withdrawal profile that varies between astarting flow rate and a se

Figure 1: Rear view of the PHD ULTRA™pump showing important connections. ...16Figure 2: Front view of the PHD ULTRA™pump with important compone

TO SET UP/EDIT A PULSE RATE PROFILE:1. From the Step Definition screen for a Pulse Rate profile, choose the Set Mode button to toggle theFlow Mode bet

BO LUS PRO FILEThe Bolus Rate profile is used to infuse a defined dose of fluid controlled either by total volume or by thetotal time the pump operate

4. On the Units Selection screen, select the unit of measure (ml, µl, nl or pl) and the unit of time (sec,min, hr) as appropriate, then choose Accept

CO NCEN TRATI ON P ROFILEThe Concentration flow profile is used to manage infusion of a defined concentration of drug or otherliquid to a set dosage l

TO SET UP/EDIT A CONCENTRATION FLOW PROFILE:1. From the Step Definition screen for a Concentration profile, choose the Animal Weight button to enterth

13. When all parameters on the Set Dose or Duration screen are correct, choose the Accept button toreturn to the Step Definition screen.14. Choose the

AU TOFILL PROF ILEThe Autofill flow profile is an automated infuse/withdraw or withdraw/infuse step that operates atconstant, but separately defined,

TO SET UP/EDIT AN AUTOFILL FLOW PROFILE:1. From the Step Definition screen for an Autofill profile, choose the Set mode button to toggle betweenthe Wi

Incorporating Advanced Options (Commands and I/O) Into A MethodPHD ULTRA™Methods can incorporate a variety of commands that expand the versatility of

GO TO STE PThe Go To Command is used to instruct the PHD ULTRA™to jump to a particular Step in a Method. Thiscan be used to help you loop back or j

Figure 27: Method Selection screen showing a listing of Methods in an Infuse/Withdraw Programmable model PHD ULTRA™pump. ...

DE LAYThe Delay Command is used to insert a time delay in a procedure. You can program a time delay between0.2 seconds up to 99:99:99 (99 hours, 99 m

RE PEAT STEPThe Repeat Command is used to repeat Steps in a procedure a defined number of times. To use thisCommand, you will tell the application wh

LI NK METH ODThe Link Command is used to insert a link to another stored Method into the operating sequence of aMethod. You can Link a Method anyplac

I/ O E VENT TRIGGE RSThe PHD Ultra pump permits easy connection to external I/O devices via a variety of interfaces, including abuilt-in 15-pin D-sub

TO INSERT AN I/O TRIGGER (OUT) COMMAND:1. From the Method Main screen, choose Step Definition to enter the Method Steps Summary screen.2. Highlight (s

I/ O E VENT (IN)The I/O Event (in) command is used to have the pump pause and wait for a signal from an external deviceor for a manual user input at

I/ O O N EV ENT (IN )The I/O On Event (in) command is used to have the pump jump to a linked method if the signal isreceived. These events are communi

MANAGING EZ PRO METHODSAll Preloaded and User-Defined Methods are stored in the Method List for ongoing use. Over time, you maywant to manage previou

Appending A MethodFollow these instructions to Append a previously created Method into a new Method:1. From the Quick Start or Method Main screen, cho

PHD Ultra Method Export and ImportThe PHD Ultra pump permits the chaining of multiple pumps, as well as integration with/control by anexternal compute

Figure 59: Step Definition screen for a Concentration profile. ...63Figure 60: Set Animal weight screen i

EXPORTING A METHOD TO AN EXTERNAL COMPUTERFollow these instructions to export a Method to an external computer.1. Connect the pump to the external com

EXTERNAL PUMP CONTROLThis section of the PHD Ultra manual describes the control of the pump using an external computer device. Setting Up HyperTermina

5. Verify the Settings are as shown above.6. Choose ASCII Setup and select “Echo typed characters locally”then click OK to complete the setup.Harvard

PUMP CHAIN COMMANDSThe Pump Chain commands allow all pump control information to be managed from an external computersource. These commands can also

dimSets or displays the backlight level in percent. Valid range is 0 to 100. Note that specifying 0 will turn thebacklight off so that the screen wi

Run CommandsirunRuns the pump in the infuse direction.irunwrunRuns the pump in the withdraw direction.wrunrrunRuns the pump in the opposite direction.

Volume CommandscivolumeClears the infused volume.civolumectvolumeClears the target volume.ctvolumecvolumeClears both the infused and withdrawn volumes

Time CommandscitimeClears the infused time.citimectimeClears both the infused and withdrawn times.ctimecttimeClears the target time.cttimecwtimeClears

Internal CommandsInternal commands are used by the pump but are not meant to be entered by the user. They are listed hereas additional information.me

AppendicesAPPENDIX A: SYRINGE VOLUME/DIAMETER REFERENCE TABLEHarvard ApparatusStainless SteelSize Diameter2.5 ml 4.851 mm8 9.52520 19.1350 28.6100 34

Figure 93: Daisy-Chaining with Legacy RJ-11 Option. ...108Figure 94: RS-485 In/Out - IEEE-139

APPENDIX B: STAINLESS STEEL SYRINGESHHaarrvvaarrdd HHiigghh PPrreessssuurree SSttaaiinnlleessss SStteeeell SSyyrriinnggeessHarvard offers a comp

APPENDIX C: MIN/MAX FLOW RATESHarvard Apparatus PHD ULTRA™Syringe Pump Series User's ManualPublication 5419-002-Rev-A91AppendicesNominal Minimum

APPENDIX D: SYRINGE HOLDER OPTIONSMounting a 4x140 Multi-Rack Figure 74: 4x140 Multi-Rack Syringe Holder Assembly.1. Remove Syringe Clamp bracket from

Figure 75: 4x140 Multi-Rack Pusher Block Assembly.1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing the Bracket Clamping Knob completely andre

Mounting a 6x10 Multi-RackFigure 76: 6x10 Multi-Rack Syringe Holder Assembly.1. Remove Syringe Clamp bracket from the Syringe Holder by unscrewing kno

Figure 77: 6x10 Multi-Rack Pusher Block Assembly.1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing the Bracket Clamping Knob completely andrem

Syringe Loading – 4 x 140 & 6 x 10 Multi-Racks Figure 78: Front view of PHD ULTRA™showing controls for multi-rack syringe loading.1. Raise or remo

11. Squeeze the Release Tabs (1) and move the Pusher Block (2) to the left until the plunger is located justto the left of the maximum volume allowabl

Mounting a Micro Liter Multi-RackFigure 80: Micro Liter Multi-Rack Syringe Holder Assembly.1. Remove Syringe Clamp and Knob from the Syringe Holder.2.

Figure 81: Micro Liter Multi-Rack Pusher Block Assembly.5. Remove the Pusher Blocks Retaining Bracket by unscrewing the Bracket Clamping Knob complet