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Agilent TechnologiesAgilent 1290 Infinity LC SystemSystem Manual and Quick Reference

10 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyTheory of Using Smaller Par

100 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Acquisition in Method and Run Control ViewRunning the Method for

Agilent 1290 Infinity LC System Manual and Quick Reference 101Quick Start Guide5Data Acquisition in Method and Run Control ViewRunning the method fast

102 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Acquisition in Method and Run Control ViewThis separation is act

Agilent 1290 Infinity LC System Manual and Quick Reference 103Quick Start Guide5Data AnalysisData AnalysisA method in the ChemStation contains all the

104 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData AnalysisData Analysis ViewTo open a chromatogram in the Data Ana

Agilent 1290 Infinity LC System Manual and Quick Reference 105Quick Start Guide5Data AnalysisIntegrating a Signal1 Select the Integration Task Tool (s

106 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Analysis8 Exit from the Events Table using the green tick icon (

Agilent 1290 Infinity LC System Manual and Quick Reference 107Quick Start Guide5Data AnalysisSpecify the Report1 On the menu bar click Report > Spe

108 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Analysis2 With the example settings shown in the figures above y

109Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies6AppendixSafety Information 110Solvent Information 113Agilent Technol

Agilent 1290 Infinity LC System Manual and Quick Reference 11Introduction to Ultra-High Performance Liquid Chromatography1Theory of Using Smaller Part

110 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSafety InformationSafety InformationGeneral Safety Information The following g

Agilent 1290 Infinity LC System Manual and Quick Reference 111Appendix6Safety Informationthe instrument must be made inoperative and be secured agains

112 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSafety InformationSafety SymbolsTabl e 1 0 Safety SymbolsSymbol DescriptionThe

Agilent 1290 Infinity LC System Manual and Quick Reference 113Appendix6Solvent InformationSolvent InformationFlow Cell To protect optimal functionalit

114 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixAgilent Technologies on InternetAgilent Technologies on InternetFor the latest

Agilent 1290 Infinity LC System Manual and Quick Reference 115Appendix6Setting Up a Method using Edit Entire MethodSetting Up a Method using Edit Enti

116 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodThis dialog summarizes the section

Agilent 1290 Infinity LC System Manual and Quick Reference 117Appendix6Setting Up a Method using Edit Entire MethodMethod InformationThe Method Inform

118 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodInstrument/AcquisitionSetup Instru

Agilent 1290 Infinity LC System Manual and Quick Reference 119Appendix6Setting Up a Method using Edit Entire MethodAutosampler tab (HiP-ALS)Figure 37

12 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyTheory of Using Smaller Par

120 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire Method• Location determines which vial o

Agilent 1290 Infinity LC System Manual and Quick Reference 121Appendix6Setting Up a Method using Edit Entire MethodThis reduces the delay volume of th

122 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodHigh Performance Autosampler (Hip_

Agilent 1290 Infinity LC System Manual and Quick Reference 123Appendix6Setting Up a Method using Edit Entire MethodBinary Pump (BinPump) tabFigure 39

124 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire Methodchannels, for example A2 and B1; i

Agilent 1290 Infinity LC System Manual and Quick Reference 125Appendix6Setting Up a Method using Edit Entire Method•Stop Time defines the overall time

126 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodThermostatted Column Compartment (

Agilent 1290 Infinity LC System Manual and Quick Reference 127Appendix6Setting Up a Method using Edit Entire MethodThe temperature of each zone can be

128 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodDiode-Array Detector (DAD) tabFigu

Agilent 1290 Infinity LC System Manual and Quick Reference 129Appendix6Setting Up a Method using Edit Entire Methodanalysis stops before the end of th

Agilent 1290 Infinity LC System Manual and Quick Reference 13Introduction to Ultra-High Performance Liquid Chromatography1Theory of Using Smaller Part

130 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire Method• Store controls the spectral coll

Agilent 1290 Infinity LC System Manual and Quick Reference 131Appendix6Setting Up a Method using Edit Entire MethodThe entrance slit to the spectrogra

132 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodInstrument Curves TabFigure 42 Set

Agilent 1290 Infinity LC System Manual and Quick Reference 133Appendix6Setting Up a Method using Edit Entire MethodData AnalysisSignal DetailsThe Sign

134 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodFigure 43 Signal details

Agilent 1290 Infinity LC System Manual and Quick Reference 135Appendix6Setting Up a Method using Edit Entire MethodEdit Integration Events The Edit In

136 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodThe Edit Integration Events screen

Agilent 1290 Infinity LC System Manual and Quick Reference 137Appendix6Setting Up a Method using Edit Entire MethodFigure 45 Specify Report ScreenTo s

138 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodOn the Reporting settings tab:•Rep

Agilent 1290 Infinity LC System Manual and Quick Reference 139Appendix6Setting Up a Method using Edit Entire MethodInstrument CurvesFigure 46 Instrume

14 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyBenefits of Columns Packed

140 Agilent 1290 Infinity LC System Manual and Quick Reference6 AppendixSetting Up a Method using Edit Entire MethodRun Time Checklist The Run Time Ch

Agilent 1290 Infinity LC System Manual and Quick Reference 141Appendix6Setting Up a Method using Edit Entire MethodThe access points in the work flow

142 Agilent 1290 Infinity LC System Manual and Quick ReferenceIndexIndexAAgilent 1290 Infinity LC Systemnew features 22power range 22system Componen

Agilent 1290 Infinity LC System Manual and Quick Reference 143IndexOonline plotconfiguring 93optimizationachieving high throughput 53achieving higher

Agilent Technologies 2009-2011, 2012Printed in Germany 05/2012*G4220-90301**G4220-90301*G4220-90301www.agilent.comAgilent TechnologiesIn This BookTh

Agilent 1290 Infinity LC System Manual and Quick Reference 15Introduction to Ultra-High Performance Liquid Chromatography1Benefits of Columns Packed W

16 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyBenefits of Columns Packed

Agilent 1290 Infinity LC System Manual and Quick Reference 17Introduction to Ultra-High Performance Liquid Chromatography1Benefits of Columns Packed W

18 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyFrictional HeatingFrictiona

Agilent 1290 Infinity LC System Manual and Quick Reference 19Introduction to Ultra-High Performance Liquid Chromatography1Frictional HeatingFigure 9 I

Agilent 1290 Infinity LC System Manual and Quick ReferenceNotices© Agilent Technologies, Inc. 2009-2011, 2012No part of this manual may be reproduced

20 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyFrictional Heating

21Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies2The Agilent 1290 Infinity LC System - Product Description New Feature

22 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionNew Features of the Agilent 12

Agilent 1290 Infinity LC System Manual and Quick Reference 23The Agilent 1290 Infinity LC System - Product Description2New Features of the Agilent 129

24 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionNew Features of the Agilent 12

Agilent 1290 Infinity LC System Manual and Quick Reference 25The Agilent 1290 Infinity LC System - Product Description2System ComponentsSystem Compone

26 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsFigure 12 Par

Agilent 1290 Infinity LC System Manual and Quick Reference 27The Agilent 1290 Infinity LC System - Product Description2System Componentschromatographi

28 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsThe flow path

Agilent 1290 Infinity LC System Manual and Quick Reference 29The Agilent 1290 Infinity LC System - Product Description2System ComponentsThe 1290 Infin

Agilent 1290 Infinity LC System Manual and Quick Reference 3In This Guide...In This Guide...This manual covers the Agilent 1290 Infinity LC System.1 I

30 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsTabl e 2 Valv

Agilent 1290 Infinity LC System Manual and Quick Reference 31The Agilent 1290 Infinity LC System - Product Description2System ComponentsThe standard a

32 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsThe Agilent 1

Agilent 1290 Infinity LC System Manual and Quick Reference 33The Agilent 1290 Infinity LC System - Product Description2System ComponentsA totally new

34 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsThe Agilent 1

Agilent 1290 Infinity LC System Manual and Quick Reference 35The Agilent 1290 Infinity LC System - Product Description2System ComponentsFigure 19 Quic

36 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem ComponentsThe 1290 Infi

Agilent 1290 Infinity LC System Manual and Quick Reference 37The Agilent 1290 Infinity LC System - Product Description2System ComponentsThe module als

38 Agilent 1290 Infinity LC System Manual and Quick Reference2 The Agilent 1290 Infinity LC System - Product DescriptionSystem Components

39Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies3Optimization of the Agilent 1290 Infinity LC SystemDelay Volume and E

4 Agilent 1290 Infinity LC System Manual and Quick ReferenceContentsContents1 Introduction to Ultra-High Performance Liquid Chromatography 7Theory of

40 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemDelay Volume and Extra-Column Volume

Agilent 1290 Infinity LC System Manual and Quick Reference 41Optimization of the Agilent 1290 Infinity LC System3Delay Volume and Extra-Column Volumet

42 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Configure the Optimum Delay V

Agilent 1290 Infinity LC System Manual and Quick Reference 43Optimization of the Agilent 1290 Infinity LC System3How to Configure the Optimum Delay Vo

44 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Configure the Optimum Delay V

Agilent 1290 Infinity LC System Manual and Quick Reference 45Optimization of the Agilent 1290 Infinity LC System3How to Configure the Optimum Delay Vo

46 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Configure the Optimum Delay V

Agilent 1290 Infinity LC System Manual and Quick Reference 47Optimization of the Agilent 1290 Infinity LC System3How to Configure the Optimum Delay Vo

48 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Configure the Optimum Delay V

Agilent 1290 Infinity LC System Manual and Quick Reference 49Optimization of the Agilent 1290 Infinity LC System3How to Configure the Optimum Delay Vo

Agilent 1290 Infinity LC System Manual and Quick Reference 5Contents6 Appendix 109Safety Information 110Solvent Information 113Agilent Technologies on

50 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Configure the Optimum Delay V

Agilent 1290 Infinity LC System Manual and Quick Reference 51Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher Injection Volum

52 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher Injection Volu

Agilent 1290 Infinity LC System Manual and Quick Reference 53Optimization of the Agilent 1290 Infinity LC System3How to Achieve High ThroughputHow to

54 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve High ThroughputThe in

Agilent 1290 Infinity LC System Manual and Quick Reference 55Optimization of the Agilent 1290 Infinity LC System3How to Achieve High ThroughputFigure

56 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher ResolutionHow

Agilent 1290 Infinity LC System Manual and Quick Reference 57Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher Resolutionthe d

58 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher ResolutionThe

Agilent 1290 Infinity LC System Manual and Quick Reference 59Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher SensitivityHow

6 Agilent 1290 Infinity LC System Manual and Quick ReferenceContents

60 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher SensitivityHow

Agilent 1290 Infinity LC System Manual and Quick Reference 61Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher SensitivityFor

62 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher Sensitivitybut

Agilent 1290 Infinity LC System Manual and Quick Reference 63Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher SensitivitySlit

64 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Higher SensitivityThe

Agilent 1290 Infinity LC System Manual and Quick Reference 65Optimization of the Agilent 1290 Infinity LC System3How to Achieve Higher SensitivityThe

66 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Achieve Lowest Carry OverHow

Agilent 1290 Infinity LC System Manual and Quick Reference 67Optimization of the Agilent 1290 Infinity LC System3How to Achieve Lowest Carry OverThe f

68 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Prevent Column BlockagesHow t

Agilent 1290 Infinity LC System Manual and Quick Reference 69Optimization of the Agilent 1290 Infinity LC System3How to Prevent Column Blockages9 Purg

7Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies1Introduction to Ultra-High Performance Liquid ChromatographyTheory of

70 Agilent 1290 Infinity LC System Manual and Quick Reference3 Optimization of the Agilent 1290 Infinity LC SystemHow to Prevent Column Blockages

71Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies4System Setup and InstallationInstalling Software 72Installing the Mod

72 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling SoftwareInstalling SoftwareInstalling the Softw

Agilent 1290 Infinity LC System Manual and Quick Reference 73System Setup and Installation4Installing the ModuleInstalling the ModuleFor details of in

74 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleFigure 25 Recommended stack configura

Agilent 1290 Infinity LC System Manual and Quick Reference 75System Setup and Installation4Installing the ModuleFigure 26 Recommended stack configurat

76 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleTwo Stack ConfigurationIn case the au

Agilent 1290 Infinity LC System Manual and Quick Reference 77System Setup and Installation4Installing the ModuleFigure 28 Recommended two stack config

78 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleOptimizing the Stack Configuration (Q

Agilent 1290 Infinity LC System Manual and Quick Reference 79System Setup and Installation4Installing the ModuleFigure 29 Recommended stack configurat

8 Agilent 1290 Infinity LC System Manual and Quick Reference1 Introduction to Ultra-High Performance Liquid ChromatographyTheory of Using Smaller Part

80 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleFigure 30 Recommended stack configura

Agilent 1290 Infinity LC System Manual and Quick Reference 81System Setup and Installation4Installing the ModuleTwo Stack ConfigurationIn case the aut

82 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleFigure 32 Recommended two stack confi

Agilent 1290 Infinity LC System Manual and Quick Reference 83System Setup and Installation4Installing the ModulePriming the PumpThis procedure is requ

84 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the Module1 Prepare channel A and pump head A f

Agilent 1290 Infinity LC System Manual and Quick Reference 85System Setup and Installation4Installing the ModulePurging the PumpThe purging procedure

86 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the Module1 To access the setup page for contro

Agilent 1290 Infinity LC System Manual and Quick Reference 87System Setup and Installation4Installing the Module2 In the Purge section, set the follow

88 Agilent 1290 Infinity LC System Manual and Quick Reference4 System Setup and InstallationInstalling the ModuleFlow Connections Between ModulesWhen

89Agilent 1290 Infinity LC System Manual and Quick ReferenceAgilent Technologies5Quick Start GuideAbout the Quick Start Guide 90Preparing the system 9

Agilent 1290 Infinity LC System Manual and Quick Reference 9Introduction to Ultra-High Performance Liquid Chromatography1Theory of Using Smaller Parti

90 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideAbout the Quick Start GuideAbout the Quick Start GuideThis chapter pro

Agilent 1290 Infinity LC System Manual and Quick Reference 91Quick Start Guide5Preparing the systemPreparing the systemTurning the System ONIf the sys

92 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuidePreparing the systemLoading the Default MethodThe ChemStation has a de

Agilent 1290 Infinity LC System Manual and Quick Reference 93Quick Start Guide5Preparing the systemConfiguring the Online Plot1 If the Online Plot win

94 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuidePreparing the system3 In the Available Signals box, highlight the requ

Agilent 1290 Infinity LC System Manual and Quick Reference 95Quick Start Guide5Preparing the systemPurging the PumpPurge the pump, if ...• The pump ha

96 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Acquisition in Method and Run Control ViewData Acquisition in Met

Agilent 1290 Infinity LC System Manual and Quick Reference 97Quick Start Guide5Data Acquisition in Method and Run Control ViewTabl e 9 Method paramete

98 Agilent 1290 Infinity LC System Manual and Quick Reference5 Quick Start GuideData Acquisition in Method and Run Control ViewSetting Up the MethodTh

Agilent 1290 Infinity LC System Manual and Quick Reference 99Quick Start Guide5Data Acquisition in Method and Run Control Viewb Other parameters can r